Uji Daya Hambat Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten) Steenis) terhadap Propionibacterium acnes
Abstract
Latar belakang : Binahong (A. cordifolia) mengandung senyawa Flavonoid, Alkaloid, Asam Oleanolik, Protein, Asam Askorbat, dan Saponin yang mempunyai aktivitas antibakteri. Tujuan : Untuk mengetahui aktivitas antibakteri daun Binahong (A. cordifolia) terhadap Propionibacterium acnes. Metode : Ekstrak daun Binahong (A. cordifolia) yang diperoleh dengan metode maserasi menggunakan etanol 96% kemudian dibuat dalam tiga konsentrasi yaitu konsentrasi 5% b/v, 10% b/v, 15% b/v dan kontrol negatif. Pengujian aktivitas antibakteri ekstrak daun Binahong (A. cordifolia) dilakukan dengan metode diffusion disk. Pengamatan dilakukan setelah 24 jam kemudian diukur zona hambat yang muncul. Hasil : ekstrak daun Binahong (A. cordifolia) dengan konsentrasi 5%, 10% dan 15% menampakkan zona hambat terhadap P. acnes masing-masing sebesar 12 mm, 18,66 mm dan 25,66 mm. Simpulan : Berdasarkan pengujian yang telah dilakukan, dapat disimpulkan bahwa ekstrak etanol daun Binahong (A. cordifolia) memiliki senyawa antibakteri terhadap P. acnes
References
Christensen, G. J. M., Scholz, C. F. P., Enghild, J., Rohde, H., Kilian, M., Thürmer, A., Brzuszkiewicz, E., Lomholt, H. B., Brüggemann, H. (2016). Antagonism between Staphylococcus epidermidis and Propionibacterium acnes and its genomic basis. BMC Genom. 17:152
Elya, B., Handayani, R., Sauriasari, R., Azizahwati, Hasyyati, U. S., Permana, I. T., Permatasari, Y. I. (2015). Antidiabetic activity and phytochemical screening of extracts from Indonesian plants by inhibition of alpha amylase, alpha glucosidase and dipeptidyl peptidase IV. Pakistan Journal of Biological Sciences. 18(6):279-284
Garmana, A. N., Sukandar, E. Y., Fidrianny, I. (2012). Activity of several plant extracts against drug-sensitive and drug-resistant microbes. Procedia Chemistry. 13:164-169.
Horng, C. T., Chao, H. R., Lee, C. F., Hsueh, C. W., Chen, F. A. (2012). Gastro protective effect of Madeira vine against ethanol-induced gastric mucosal lesion in rat. Asian Journal of Chemistry. 24(2):765-768.
Kumowal, S., Fatimawali, F., Jayanto, I. (2019). Uji Aktivitas Antibakteri Nanopartikel Ekstrak Lengkuas Putih (Alpinia galanga (L.) Willd) terhadap Bakteri Klebsiella pneumoniae. Pharmacon. 8(4):781-790.
Lestari, D., Sukandar, E. Y., Fidrianny, I. (2015). Anredera cordifolia leaves extract as antihyperlipidemia and endothelial fat content reducer in male Wistar rat. International Journal of Pharmaceutical and Clinical Research. 7(6):435-439.
McLaughlin, J., Watterson, S., Layton, A. M., Bjourson, A. J., Barnard, E., McDowell, A. (2019). Propionibacterium acnes and Acne Vulgaris: New Insights from the Integration of Population Genetic, Multi-Omic, Biochemical and Host-Microbe Studies. Microorganisms. 7(5):128
Pelczar, M. J., Chan, E. C. (2008) Dasar-Dasar Mikrobiologi Jilid 2. Universitas Indonesia Press. Jakarta
Shu, M., Wang, Y., Yu, J., Kuo, S., Coda, A., Jiang, Y., Gallo, R. L., Huang, C. M. (2013). Fermentation of Propionibacterium acnes, a commensal bacterium in the human skin microbiome, as skin probiotics against methicillin-resistant Staphylococcus aureus. 8(2):e55380.
Sukandar, E. Y., Ridwan, A., Sukmawan, Y. P. (2016). Vasodilatation effect of ethanolic extract of Anredera cordifolia, Sonchus arvensis L, and ursolic acid on isolated rabbit aortic and frog heart. International Journal of Pharmacy and Pharmaceutical Sciences. 8(2):145-149
Sukandar, E. Y., Kurniati, N. F., Nurdianti, A. N. (2016). Antiobesity effect of ethanol extract of Anredera cordifolia (Ten.) Steenis leaves on obese male Wistar rats induced by high-carbohydrate diet. International Journal of Pharmacy and Pharmaceutical Sciences. 8(4):171-173.
Susanti, A. (2008). Daya antibakteri ekstrak etanol daun Beluntas (Pluchea indica (L.) Less) terhadap Escherichia coli secara in vitro. Veterinaria Medika. 1(1):29-32
World Health Organization. (2021). Antimicrobial Resistance from World Health Organization. Available from: https://www.who.int/news-room/fact-sheets/detail/antimicrobial-resistance (diakses 10 September 2023)
Yuliani, S. H., Fudholi, A., Pramono, S., Marchaban. (2012). The effect of formula to physical properties of wound healing gel of ethanolic extract of binahong (Anredera cordifolia (Ten.) Steenis). International Journal of Pharmaceutical Sciences and Research. 27(3):4254-4259.
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